Three-time champion Serena Williams has withdrawn from the French Open because of an Achilles injury.
The 39-year-old had been scheduled to play her second-round match against Bulgaria’s Tsvetana Pironkova on Wednesday.
The American has won 23 Grand Slam singles titles and was aiming to equal Margaret Court’s all-time record of 24.
Williams picked up the Achilles problem during her US Open semi-final defeat by Victoria Azarenka earlier this month.
On Monday, she beat fellow American Kristie Ahn 7-6 (7-2) 6-0 in the first round at Roland Garros, but said she was struggling physically.
Williams won the French Open in 2002, 2013 and 2015 and is currently ranked ninth in the world.